Matrix-matched Calibration
Another option for mass spectrometric quantitation is the matrix-matched calibration. In this case, samples, which do not contain the analyte are spiked with respective concentration of the analytes. The obtained calibration curves are used for quantitation.
Advantage
The matrix-matched calibration corrects the ionization influence of the matrix.
Disadvantage
The preparation of the calibration curve is more labor intense. Due to the lack of an internal standard no losses during sample preparation are compensated. The matrix-matched calibration often fails due to the lack of a sample which does not contain the analyte of interest.
Literature Example