Standard Addition
Using the method of standard addition for quantitation, respective amounts of analyte are spiked to a given sample. The peak area is determined for each respective calibration point and analyzed by linear regression. The intercept of the regression line with the x-axis corresponds to the amount of analyte in the sample.
Advantage
In case the amount of a specific analyte is close to a specific threshold value, the standard addition is a valuable method to accurately quantify the analyte concentration.
Disadvantage
The calibration curve for the standard addition method has to be prepared for every single sample. Because of this, this method is quite labor intensive and uses a lot of measurement time.
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